Vitamin C60 Bioresearch, the world's first commercialization of plant growth factor "AOH" as a cosmetic raw material

 Vitamin C60 Bioresearch has developed the world's first cosmetic product through joint research with Shizuoka University's newly discovered plant growth factor 2-aza-8-oxohypoxanthine (AOH). We succeeded in commercializing it as a raw material and launched it in October this year as an aging care ingredient "Repista" that improves the skin barrier function.

This time, Vitamin C60 Bioresearch has succeeded in turning AOH into a cosmetic raw material, which is a new natural compound discovered by Dr. Hirokazu Kawagishi, Distinguished Emeritus Professor of Shizuoka University, who conducted joint research. Professor Kawagishi is a leading figure in the discovery of various bioactive substances in mushrooms, including the discovery of a pharmacologically active substance derived from Lion's Mane mushroom that has an anti-dementia effect, and the elucidation of acute encephalopathy caused by Sugihiratake. He has also scientifically elucidated a phenomenon known as the "fairy ring", which has been a mystery for many years, and discovered fairy compounds (FCs) as plant growth regulators. In May, he was awarded the Medal with Purple Ribbon.

A fairy ring is a phenomenon in which a ring of grass grows thicker than its surroundings in parks, golf courses, residential areas, etc., and mushrooms grow in its wake. ” and announced in 2014.

AOH is a growth factor possessed by many edible plants other than mushrooms, such as rice and tomato.

In the vitamin C60 bioresearch, we focused on the plant growth promoting effect of AOH and thought that it might be useful for human skin. started.

After about five years of research, they established a technology to produce AOH using enzymes secreted by microorganisms. In addition, we succeeded in making it water-soluble by using sodium hydroxide and water so that it can be easily blended in cosmetics, and we also verified its efficacy and safety. started selling.

“Lepista was named after the scientific name of Lepista sordida where AOH was discovered. AOH is a growth factor found in many plants such as rice and tomatoes, but its content is very small. For example, if only 1g can be obtained from 300 million yen worth of Koshihikari rice, it may be easy to understand its rarity.It was difficult to establish the manufacturing process for this AOH.Our company has been developing originality, as typified by fullerene. Our researcher Ohe Aoshima received an excellent presentation award at the 39th Annual Meeting and Scientific Meeting of the Japanese Society of Aesthetic Dermatology. The novelty and challenge of developing AOH, a plant growth factor, as a cosmetic raw material for the first time in the world, was highly evaluated, as well as the uniqueness of the substance itself.”

Repista's main anti-aging effects are expected to have five effects: "cell activation," "skin barrier function improvement," "turnover promotion," "hyaluronic acid production promotion (moisturizing)," and "transparency."

In the cell activation evaluation test, Repista was added to normal human epidermal keratinocytes (0.04% to 0.3%), cultured for 48 hours, and then the cell viability was measured using the MTT assay. As a result, a concentration-dependent increase in cell viability was observed, suggesting that it has an activating effect on epidermal cells.